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Related post: LMSF, NIAID COOPERATING UNITS (it any) Z. Pei, Special Volunteer K. Brown, Special Volunteer, Arthritis Foundation V. Tolstikov. Contractor LAB/BRANCH Laboratory of Microbial Structure and Function INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20892 TOTAL STAFF YEARS: 4.25 PROFESSIONAL: 2.5 1.75 CHECK APPROPRIATE BOX{ES) D (a) Human subjects n (a1) Minors D (a2) Interviews Kl (b) Human tissues D (c) Neither SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) The goal Toprol Xl Vs Metoprolol Tartrate of this work is to Toprol Xl Generic Name study the structure Toprol Xl Beta Blocker and function of the immunoglobulin molecule. The work is being performed so that this Toprol Xl Generic Recall understanding will be applied to the development of antibodies for human therapy. A. Therapeutic systems. 1. Antibodies to surface antigens of group B streptococci (GBS) have been Toprol Xl Vs Generic demonstrated to have protective efficacy in a model of neonatal sepsis. We have identified colony opacity variants of GBS and studied their interactions with antibodies and other components of the immune system. 2. The efficacy of anti-HIV antibodies Is Toprol A Beta Blocker coupled to ricin A-chain has been studied in vitro. Monoclonal and polyclonal antibodies directed against different epitopes have been tested. Biological variants of HIV that escape killing with these immunotoxins and CD4-PE40 have been Toprol Xl Generic Equivalent identified. The phenotype of the cells carrying these HIV has been studied, and the molecular mechanisms of immunotoxin escape have been evaluated. We have Cost Of Toprol Xl also constructed anti-MuLV immunotoxins, and the efficacy of anti-retroviral immunotoxins is being tested in vivo in well-studied animal systems . B. Genetically engineered antibodies. Vectors have been prepared carrying human constant region genes. These constructs Toprol Xl 50 Mg Recall contain both native and altered genes These constructs have been ligated to variable region genes from antibodies to the synthetic polypeptide (Tyr,Glu) -Ala--Lys . Antibodies were expressed and purified The antigen-binding capability of these antibodies has been tested. Well-defined immune complexes were constructed using antigens having different epitope densities. The Metoprolol Vs Toprol Xl ability of the immune complexes to interact with the complement system has being studied. 16-6 PflS 6040 (Hev. b/92) DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC Metoprolol Tartrate Vs Toprol Xl HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH Is Toprol Xl A Beta Blocker PROJECT PROJECT NUMBER ZOl AI 00549-04 PERIOD COVERED October 1, 1991, to September 30, 1992 TITLE OF PROJECT (BO characters or less. Title must lit on one line between the borders.) Pathogenesis of Infection with the Lyme Disease Spirochete, Barrel ia burgdorferi PRINCIPAL INVESTIGATOR (List other prolesskxial personnel bekm Generic For Toprol Xl the Principal Investigator.) (Name, title, laboratory, and institute affiliation) P.I.: P. A. Rosa Senior Staff Fellovi? LMSF, NIAID Others: K. L. Tilly Senior Staff Fellow LMSF, NIAID D. M. Hogan Microbiologist LMSF, NIAID COOPERATING UNITS (if any) N. Margolis, Special Volunteer, Arthritis Foundation J. Campbell, Stay In School Program A. Sadziene and A. Barbour. University of Texas at San Antonio LASmRANCH Laboratory of Microbial Structure and Function INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20892 TOTAL Generic Name For Toprol Xl STAFF YEARS: PROFESSIONAL: CHECK APPROPRIATE BOX(ES) D (a) Human subjects D (b) Human tissues E (c) Neither D (a1) Toprol Generic Equivalent Minors □ (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) Infection of humans by Toprol Xl 100 Mg Borrelia burgdorferi (Bb) can result in a multisystemic, chronic disease with Toprol Xl Coupons diverse manifestations. We are investigating B. burgdorferi at several levels in order to understand some aspects of the disease pathogenesis. These studies include genetic manipulations of the spirochete and characterization of key structural and regulatory elements 1. Molecular Characterization: Several features of Bb are being addressed. We have characterized homologous recombination between the genes encoding the major outer surface proteins (Osps) that both deletes osp gene sequences and creates chimeric Generic Name For Toprol Toprol Xl Coupon gene fusions. Bb expressing recombinant osp genes can "escape" killing by Osp-specific neutralizing antibodies. We are also investigating mechanisms of transcriptional regulation of osp gene expression. Isolating and studying genes whose expression is increased after a temperature upshift (heat shock genes) will provide information about their roles in cell growth, pathogenesis, plasmid replication and adaptation to stress. Understanding the mechanism of linear plasmid replication may yield a new target for antibiotics, in addition to shedding light on ways in which organisms manipulate their genetic material 2. Gene Transfer: Toprol Xl Vs Metoprolol In order to allow genetic analysis of Bb, a method for gene transfer into Borrelia is being developed. A derivative of the Bb 16 kb linear plasmid will be used as a vector for electroporation, allowing for maintenance of transferred DNA in a native setting. DNA encoding recombinant Osp proteins that lack neutralizing epitopes will be introduced as a selectable marker 3. cDNA Library: Extended passage of Bb in vitro eliminates its ability to infect
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